INSTRUCTIONS FOR THE USE OF THE MS10 MEMBRANE

The membrane is fragile when dry.
Do not press the dry membrane.
Do not bend the dry membrane.
Do not pull the dry membrane.
The membrane will smooth out and strengthen when floated on media.

The membrane is a rectangular strip (approx.) 2.0 x 4.0 cm. Each ‘window’ area diam. falls in the range 1.0 – 1.2 cm, and each membrane displays three such ‘windows’.

Sterilize the membrane by immersing it in 70% ethyl alcohol for one to two minutes. Place the membrane on sterile gauze or filter paper and dry it FULLY. (Do not dry it on glass or metal, it may stick and require complete wetting to release the surface).
The membrane is now ready for use.

Float the membrane on a small volume of media in a Petri dish. Avoid wetting the upper surface. Deposit a drop of cell suspension in a window area. The three windows are available for use at the same time.

Proceed with the incubation.

NOTES
Note that one surface of the membrane appears matte and the opposite surface appears shiny. This is more noticeable when the membrane is dry. While the shiny surface may function very well in supporting cell cultures, it is recommended that, in initial trials, the membranes be used with the matte surface facing up. The matte appearance is due to microscopic protrusions that cover the surface and such surface structure may serve as a scaffold for culture growth.

Sterilized,dry membranes which have been wetted accidentally with sterile water may be readied for use by simply blotting them gently between sheets of sterile gauze and then floating them on media.

Haskel Technology membranes are for laboratory and investigational use only, not for human or animal use.